Detection of murine norovirus 1 by using plaque assay, transfection assay, and real-time reverse transcription-PCR before and after heat exposure.

نویسندگان

  • Leen Baert
  • Christiane E Wobus
  • Els Van Coillie
  • Larissa B Thackray
  • Johan Debevere
  • Mieke Uyttendaele
چکیده

The correlation between the detection of murine norovirus 1 RNA by real-time reverse transcription-PCR and the infectivity by plaque assay before and after heat exposure (80 degrees C) was examined. No correlation was found in the current study. Moreover, heat inactivation had a much stronger detrimental effect on virus infectivity than on the integrity of the viral genome.

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عنوان ژورنال:
  • Applied and environmental microbiology

دوره 74 2  شماره 

صفحات  -

تاریخ انتشار 2008